| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| 7xHis | — | 6xHis affinity tag | 154..175 | Fwd | |
|
DNA Sequence
CATCACCATCACCATCACCAT
Protein Sequence
HHHHHHH
|
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| TEV site | — | tobacco etch virus (TEV) protease recog… | 199..220 | Fwd | |
|
DNA Sequence
GAGAATCTTTATTTTCAGGGA
Protein Sequence
ENLYFQG
|
|||||
| LC3A | MAP1LC3A | human LC3 protein | 223..583 | Fwd | |
|
DNA Sequence
CCCTCAGACCGGCCTTTCAAGCAGCGGCGGAGCTTCGCCGACCGCTGTAAGGAGGTACAGCAGATCCGCGACCAGCACCCCAGCAAAATCCCGGTGATCATCGAGCGCTACAAGGGTGAGAAGCAGCTGCCCGTCCTGGACAAGACCAAGTTTTTGGTCCCGGACCATGTCAACATGAGCGAGTTGGTCAAGATCATCCGGCGCCGCCTGCAGCTGAACCCCACGCAGGCCTTCTTCCTGCTGGTGAACCAGCACAGCATGGTGAGTGTGTCCACGCCCATCGCGGACATCTACGAGCAGGAGAAAGACGAGGACGGCTTCCTCTATATGGTCTACGCCTCCCAGGAAACCTTCGGCTTC
Protein Sequence
PSDRPFKQRRSFADRCKEVQQIRDQHPSKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELVKIIRRRLQLNPTQAFFLLVNQHSMVSVSTPIADIYEQEKDEDGFLYMVYASQETFGF
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| Shine-Dalgarno sequence | — | — | 270..279 | Fwd | |
|
DNA Sequence
TAAGGAGGT
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T5 promoter | — | — | 43..88 | Fwd | |
|
DNA Sequence
TCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATA
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lac operator | — | — | 63..80 | Fwd | |
|
DNA Sequence
TTGTGAGCGGATAACAA
|
|||||
| lac operator | — | — | 95..112 | Fwd | |
|
DNA Sequence
TTGTGAGCGGATAACAA
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lambda t0 terminator | — | — | 628..723 | Fwd | |
|
DNA Sequence
GACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAAT
|
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