His-PIMT

34852 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Bruce Downie
Article
Dinkins et al Plant J. 2008 Jul;55(1):1-13.
Insert
PIMT (PCMT1 Human)
Tags
6XHis
Promoter
T7
Available Since
Jan. 24, 2012
Availability
Available

Sequence Information

Length
570 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 37..55 Fwd
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 64..82 Fwd
DNA Sequence
CTGGTGCCACGCGGTTCT
Protein Sequence
LVPRGS
S-Tag — affinity and epitope tag derived from p… 88..133 Fwd
DNA Sequence
AAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGC
Protein Sequence
KETAAAKFERQHMDS
enterokinase site — enterokinase recognition and cleavage s… 148..163 Fwd
DNA Sequence
GACGACGACGACAAG
Protein Sequence
DDDDK