BRCA1-(360-498)

35068 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Cheryl Arrowsmith
Article
Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.
Insert
BRCA1 (BRCA1 Human)
Tags
His
Mutation
Contains aa 498-360
Promoter
T7
Available Since
April 4, 2012
Availability
Available

Sequence Information

Length
891 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 55..73 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 82..100 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
promoter 1
Label Gene Product Position Strand
tet promoter — — 812..841 Rev
DNA Sequence
TTGACAGCTTATCATCGATAAGCTTTAAT
terminator 1
Label Gene Product Position Strand
T7 terminator — — 590..638 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG