BRCA1-(408-540)

35069 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Cheryl Arrowsmith
Article
Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.
Insert
BRCA1 (BRCA1 Human)
Tags
His
Mutation
Contains aa 408-540
Promoter
T7
Available Since
April 4, 2012
Availability
Available

Sequence Information

Length
921 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 59..77 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 86..104 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
promoter 1
Label Gene Product Position Strand
tet promoter — — 798..827 Rev
DNA Sequence
TTGACAGCTTATCATCGATAAGCTTTAAT
terminator 1
Label Gene Product Position Strand
T7 terminator — — 576..624 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG