DOT1L

36196 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
DOT1L (GeneScript Synthesized) (DOT1L Human)
Tags
His tag with TEV cleavage
Mutation
contains amino acid residues 1-420
Available Since
Aug. 8, 2012
Availability
Available

Sequence Information

Length
930 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 40..58 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 70..91 Fwd
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG