RFXANK (3V3O)

36905 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
RFXANK (RFXANK Human)
Tags
His tag with TEV cleavage
Mutation
amino acid residues 90-260 (ANK repeats)
Available Since
June 19, 2012
Availability
Available

Sequence Information

Length
874 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 37..55 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 67..88 Fwd
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG
6xHis — 6xHis affinity tag 630..648 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 714..763 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG