MAGI1

38883 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Nicola Burgess-Brown
Insert
MAGI1 (MAGI1 Human)
Tags
His Tev
Mutation
C1189S; codon-optimized
Available Since
March 18, 2013
Availability
Available

Sequence Information

Length
860 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 18..36 Rev
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 371..392 Rev
DNA Sequence
GAGAACCTGTACTTCCAATCC
Protein Sequence
ENLYFQS
6xHis — 6xHis affinity tag 416..434 Rev
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 506..525 Rev
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 481..506 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC