PTPN4 — PlasmidDB

Plasmid Information

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

PTPN4 (PTPN4 Human)

Length

836 bp

Topology

linear

Molecule Type

genomic DNA

CDS 2

Label	Gene	Product	Position	Strand
TEV site	—	tobacco etch virus (TEV) protease recog…	390..411	Rev
DNA Sequence GAGAACCTGTACTTCCAATCC Protein Sequence ENLYFQS
6xHis	—	6xHis affinity tag	435..453	Rev
DNA Sequence CACCATCATCATCATCAT Protein Sequence HHHHHH

promoter 1

Label	Gene	Product	Position	Strand
T7 promoter	—	—	525..544	Rev
DNA Sequence TAATACGACTCACTATAGG

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	500..525	Fwd
DNA Sequence GGAATTGTTATCCGCTCACAATTCC