| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| TEV site | — | tobacco etch virus (TEV) protease recog… | 810..831 | Fwd | |
|
DNA Sequence
GAGAACCTCTACTTCCAATCG
Protein Sequence
ENLYFQS
|
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| 6xHis | — | 6xHis affinity tag | 831..849 | Fwd | |
|
DNA Sequence
CACCATCATCACCACCAT
Protein Sequence
HHHHHH
|
|||||
| FLAG | — | FLAG(R) epitope tag, followed by an ent… | 849..873 | Fwd | |
|
DNA Sequence
GATTACAAGGATGACGACGATAAG
Protein Sequence
DYKDDDDK
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| Shine-Dalgarno sequence | — | — | 743..752 | Fwd | |
|
DNA Sequence
ACCTCCTTA
|
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