| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| TEV site | — | tobacco etch virus (TEV) protease recog… | 767..788 | Fwd | |
|
DNA Sequence
GAGAACCTCTACTTCCAATCG
Protein Sequence
ENLYFQS
|
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| 6xHis | — | 6xHis affinity tag | 788..806 | Fwd | |
|
DNA Sequence
CACCATCATCACCACCAT
Protein Sequence
HHHHHH
|
|||||
| FLAG | — | FLAG(R) epitope tag, followed by an ent… | 806..830 | Fwd | |
|
DNA Sequence
GATTACAAGGATGACGACGATAAG
Protein Sequence
DYKDDDDK
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T7 terminator | — | — | 966..1013 | Fwd | |
|
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAACGGGTCTTGAGGGGTTTTTTG
|
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