| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| 6xHis | — | 6xHis affinity tag | 145..163 | Fwd | |
|
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
|
|||||
| TrxA | trxA | E. coli thioredoxin | 172..499 | Fwd | |
|
DNA Sequence
ATGAGCGATAAAATTATTCACCTGACTGACGACAGTTTTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGCGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCC
Protein Sequence
MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLA
|
|||||
| TEV site | — | tobacco etch virus (TEV) protease recog… | 505..526 | Fwd | |
|
DNA Sequence
GAGAACTTGTACTTCCAATCC
Protein Sequence
ENLYFQS
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T7 promoter | — | — | 54..73 | Fwd | |
|
DNA Sequence
TAATACGACTCACTATAGG
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lac operator | — | — | 73..98 | Fwd | |
|
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
|
|||||