TP73

39077 Homo sapiens Bacterial Expression

Plasmid Information

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

TP73 (TP73 Human)

Tags

TEV-His6-Flag

Available Since

March 18, 2013

Availability

Available

Sequence Information

Length

942 bp

Topology

linear

Molecule Type

genomic DNA

View on AddGene.org

Features

CDS 4

Label	Gene	Product	Position	Strand
TEV site	—	tobacco etch virus (TEV) protease recog…	747..768	Fwd
DNA Sequence GAGAACCTCTACTTCCAATCG Protein Sequence ENLYFQS
6xHis	—	6xHis affinity tag	768..786	Fwd
DNA Sequence CACCATCATCACCACCAT Protein Sequence HHHHHH
FLAG	—	FLAG(R) epitope tag, followed by an ent…	786..810	Fwd
DNA Sequence GATTACAAGGATGACGACGATAAG Protein Sequence DYKDDDDK
6xHis	—	6xHis affinity tag	859..877	Fwd
DNA Sequence CACCACCACCACCACCAC Protein Sequence HHHHHH

promoter 1

Label	Gene	Product	Position	Strand
T7 promoter	—	—	54..73	Fwd
DNA Sequence TAATACGACTCACTATAGG

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	73..98	Fwd
DNA Sequence GGAATTGTGAGCGGATAACAATTCC