GUCY1B3

39121 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Nicola Burgess-Brown
Insert
GUCY1B3 (GUCY1B1 Human)
Tags
TEV-His6-Flag
Mutation
This is a complex of two proteins; GUCY1A3 (aa 46…
Available Since
March 18, 2013
Availability
Available

Sequence Information

Length
950 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 4
Label Gene Product Position Strand
TEV site — tobacco etch virus (TEV) protease recog… 782..803 Fwd
DNA Sequence
GAGAACCTCTACTTCCAATCG
Protein Sequence
ENLYFQS
6xHis — 6xHis affinity tag 803..821 Fwd
DNA Sequence
CACCATCATCACCACCAT
Protein Sequence
HHHHHH
FLAG — FLAG(R) epitope tag, followed by an ent… 821..845 Fwd
DNA Sequence
GATTACAAGGATGACGACGATAAG
Protein Sequence
DYKDDDDK
6xHis — 6xHis affinity tag 894..912 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 53..72 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 72..97 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC