pGEX4T-1_OCT4_T234E_S235E

40635 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
James Thomson
Article
Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109
Insert
POU5F1 (POU5F1 Human)
Tags
GST, TEV Cleavage, and Thrombin Cleavage
Mutation
T234E_S235E
Promoter
tac
Available Since
July 26, 2013
Availability
Available

Sequence Information

Length
443 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
thrombin site — thrombin recognition and cleavage site 17..35 Fwd
DNA Sequence
CTGGTTCCGCGTGGATCT
Protein Sequence
LVPRGS
TEV site — tobacco etch virus (TEV) protease recog… 41..62 Fwd
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG