NNMT

40734 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
NNMT (NNMT Human)
Tags
His Thrombin
Mutation
K100A:E101A:E103A
Available Since
Oct. 18, 2012
Availability
Available

Sequence Information

Length
949 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 62..80 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 89..107 Fwd
DNA Sequence
CTGGTTCCGCGTGGTAGT
Protein Sequence
LVPRGS
6xHis — 6xHis affinity tag 928..946 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH