ITPAA

42345 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Nicola Burgess-Brown
Insert
ITPAA (ITPA Human)
Tags
His6-Thrombin
Mutation
See Comments
Available Since
March 18, 2013
Availability
Available

Sequence Information

Length
954 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 59..77 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 86..104 Fwd
DNA Sequence
CTGGTTCCGCGTGGTAGT
Protein Sequence
LVPRGS
6xHis — 6xHis affinity tag 715..733 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH