NUDT3A

42348 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Nicola Burgess-Brown
Insert
NUDT3A (NUDT3 Human)
Tags
His6-TEV
Mutation
See Comments
Available Since
March 18, 2013
Availability
Available

Sequence Information

Length
851 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 30..48 Rev
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 557..578 Rev
DNA Sequence
GAGAACCTGTACTTCCAATCC
Protein Sequence
ENLYFQS
6xHis — 6xHis affinity tag 602..620 Rev
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 692..711 Rev
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 667..692 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC