GARTA

42391 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Nicola Burgess-Brown
Insert
GARTA (GART Human)
Tags
His6-TEV
Mutation
See Comments
Available Since
April 1, 2013
Availability
Available

Sequence Information

Length
801 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 150..168 Fwd
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 192..213 Fwd
DNA Sequence
GAGAACCTGTACTTCCAATCC
Protein Sequence
ENLYFQS
promoter 1
Label Gene Product Position Strand
T7 promoter — — 59..78 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 78..103 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC