pHisAlix deltaPRD

42577 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
James Hurley
Article
Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10
Insert
delta-PRD ALIX (residues 1-702) (PDCD6IP Human)
Tags
HIS-TEV
Mutation
silent mutation A1617G (numbering in ALIX gene)
Available Since
Dec. 19, 2013
Availability
Available

Sequence Information

Length
933 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 61..79 Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 100..121 Fwd
DNA Sequence
GAAAACCTGTATTTTCAGGGC
Protein Sequence
ENLYFQG