| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| 6xHis | — | 6xHis affinity tag | 144..162 | Fwd | |
|
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
|
|||||
| thrombin site | — | thrombin recognition and cleavage site | 171..189 | Fwd | |
|
DNA Sequence
CTGGTGCCACGCGGTTCT
Protein Sequence
LVPRGS
|
|||||
| S-Tag | — | affinity and epitope tag derived from p… | 195..240 | Fwd | |
|
DNA Sequence
AAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGC
Protein Sequence
KETAAAKFERQHMDS
|
|||||
| enterokinase site | — | enterokinase recognition and cleavage s… | 255..270 | Fwd | |
|
DNA Sequence
GACGACGACGACAAG
Protein Sequence
DDDDK
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T7 promoter | — | — | 53..72 | Fwd | |
|
DNA Sequence
TAATACGACTCACTATAGG
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lac operator | — | — | 72..97 | Fwd | |
|
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
|
|||||