pET29b_Qcat4

45132 Synthetic Bacterial Expression

Plasmid Information

Depositor
Christopher Petzold
Article
Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1
Insert
E-coli-pathways-QconCAT4
Tags
6XHis
Promoter
T7
Available Since
Dec. 12, 2013
Availability
Available

Sequence Information

Length
847 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
S-Tag — affinity and epitope tag derived from p… 137..182 Fwd
DNA Sequence
AAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGC
Protein Sequence
KETAAAKFERQHMDS
thrombin site — thrombin recognition and cleavage site 197..215 Fwd
DNA Sequence
CTGGTGCCACGCGGTTCC
Protein Sequence
LVPRGS
promoter 1
Label Gene Product Position Strand
T7 promoter — — 46..65 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 65..90 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC