pGEX-4T-1-p19-T7

46306 Synthetic Bacterial Expression

Plasmid Information

Depositor
Judy Lieberman
Article
Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do
Tags
6XHis and GST
Promoter
Tac
Available Since
July 23, 2013
Availability
Available

Sequence Information

Length
805 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
thrombin site — thrombin recognition and cleavage site 9..27 Fwd
DNA Sequence
CTGGTTCCGCGTGGATCC
Protein Sequence
LVPRGS
6xHis — 6xHis affinity tag 543..561 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 567..586 Fwd
DNA Sequence
TAATACGACTCACTATAGG