pGEX-4T-1-p19-T7-EGFPFL

46310 Synthetic Bacterial Expression

Plasmid Information

Depositor
Judy Lieberman
Article
Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do
Tags
6XHis and GST
Available Since
Jan. 2, 2014
Availability
Available

Sequence Information

Length
606 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
thrombin site — thrombin recognition and cleavage site 12..30 Fwd
DNA Sequence
CTGGTTCCGCGTGGATCC
Protein Sequence
LVPRGS
6xHis — 6xHis affinity tag 546..564 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 570..589 Fwd
DNA Sequence
TAATACGACTCACTATAGG