pAC4 Frk-SH2

46428 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Bruce Mayer
Article
Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.
Insert
Frk (FRK Human)
Tags
AviTag, GST, and Thrombin site
Mutation
contains amino acids 108-213
Promoter
Trc
Available Since
July 10, 2013
Availability
Available

Sequence Information

Length
742 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
thrombin site — thrombin recognition and cleavage site 6..24 Fwd
DNA Sequence
CTGGTTCCGCGTGGATCC
Protein Sequence
LVPRGS
AviTag(TM) — peptide tag that allows for enzymatic b… 360..405 Fwd
DNA Sequence
GGTCTGAACGACATCTTCGAGGCTCAGAAAATCGAATGGCACGAA
Protein Sequence
GLNDIFEAQKIEWHE
terminator 1
Label Gene Product Position Strand
rrnB T1 terminator Escherichia coli rrnB — 624..711 Fwd
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAAT