pGEX-5x3-hMeCP2-delN256-G273X

48090 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression of GST tagged AT-Hook2-G273X
Depositor
Huda Zoghbi
Article
Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10
Insert
Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
Tags
GST
Mutation
deleted the N-terminal 256 codons, expresses AT-H…
Available Since
April 17, 2014
Availability
Available

Sequence Information

Length
917 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
Factor Xa site — Factor Xa recognition and cleavage site 9..21 Fwd
DNA Sequence
ATCGAAGGTCGT
Protein Sequence
IEGR
7xHis — 6xHis affinity tag 366..387 Fwd
DNA Sequence
CACCACCACCATCACCACCAC
Protein Sequence
HHHHHHH
Myc — Myc (human c-Myc proto-oncogene) epitop… 732..762 Fwd
DNA Sequence
GAACAGAAACTGATCTCTGAAGAAGACCTG
Protein Sequence
EQKLISEEDL
6xHis — 6xHis affinity tag 762..780 Fwd
DNA Sequence
CATCATCACCATCACCAC
Protein Sequence
HHHHHH