pNIC28-hKdm2b-CxxC-PHD

49216 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression of human Kdm2b CxxC-PHD domain with N-His6-TEV and C-Avi tags. For use in BioCAP non-methylated DNA purification.
Depositor
Rob Klose
Insert
Kdm2b (KDM2B Human)
Tags
Avi and His6-TEV
Promoter
T7
Available Since
Dec. 18, 2013
Availability
Available

Sequence Information

Length
872 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 4
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 36..54 Fwd
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 78..99 Fwd
DNA Sequence
GAGAACCTGTACTTCCAATCC
Protein Sequence
ENLYFQS
AviTag(TM) — peptide tag that allows for enzymatic b… 561..606 Fwd
DNA Sequence
GGTCTGAACGACATCTTCGAGGCTCAGAAAATCGAATGGCACGAA
Protein Sequence
GLNDIFEAQKIEWHE
6xHis — 6xHis affinity tag 671..689 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 755..803 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG