pET28a-TRBP

50351 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
bacterial expression of TRBP
Depositor
Kumiko Ui-Tei
Article
Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1
Insert
TRBP (TARBP2 Human)
Tags
His and Myc
Promoter
T7
Available Since
Jan. 15, 2014
Availability
Available

Sequence Information

Length
893 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 125..143 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 152..170 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
promoter 1
Label Gene Product Position Strand
T7 promoter — — 26..45 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 45..70 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC