pET28a-PACT

50352 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
bacterial expression of PACT
Depositor
Kumiko Ui-Tei
Article
Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1
Insert
PACT (PRKRA Human)
Tags
His and Myc
Promoter
T7
Available Since
Jan. 15, 2014
Availability
Available

Sequence Information

Length
935 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 149..167 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 176..194 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
promoter 1
Label Gene Product Position Strand
T7 promoter — — 50..69 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 69..94 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC