| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| minicistron | — | synthetic cistron containing a ribosome… | 106..133 | Fwd | |
|
DNA Sequence
ATGTATCGATTAAATAAGGAGGAATAA
Protein Sequence
MYRLNKEE
|
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| 6xHis | — | 6xHis affinity tag | 148..166 | Fwd | |
|
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
|
|||||
| T7 tag (gene 10 leader) | — | leader peptide from bacteriophage T7 ge… | 169..202 | Fwd | |
|
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
|
|||||
| TEV site | — | tobacco etch virus (TEV) protease recog… | 214..235 | Fwd | |
|
DNA Sequence
GAAAACCTGTATTTTCAGGGC
Protein Sequence
ENLYFQG
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| rrnB T1 terminator | Escherichia coli rrnB | — | 668..755 | Fwd | |
|
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAAT
|
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