4IIO

51326 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Cheryl Arrowsmith
Insert
4IIO (ITSN2 Human, 4IIO)
Tags
His
Mutation
Truncation: See Comments
Promoter
T7
Available Since
Feb. 24, 2014
Availability
Available

Sequence Information

Length
859 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 45..63 Fwd
DNA Sequence
CACCATCACCATCACCAT
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 75..96 Fwd
DNA Sequence
GAAAATCTGTACTTCCAAGGT
Protein Sequence
ENLYFQG
terminator 1
Label Gene Product Position Strand
fd terminator — — 297..345 Fwd
DNA Sequence
ACCGATACAATTAAAGGCTCCTTTTGGAGCCTTTTTTTTGGAGATTTT