Human Phosphomevalonate kinase (PMK)

52430 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
bacterial expression of human Phosphomevalonate kinase (PMK)
Depositor
Henry Miziorko
Article
Herdendorf et al Biochemistry. 2006 Mar 14;45(10):3235-42
Insert
Phosphomevalonate kinase (PMVK Human)
Tags
His
Promoter
T7
Available Since
May 9, 2014
Availability
Available

Sequence Information

Length
780 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 147..165 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 174..192 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
promoter 1
Label Gene Product Position Strand
T7 promoter — — 48..67 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 67..92 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC