pGEX6P1-HA-MEK1 CBM

53163 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expresses GST-HA tagged human MEK1 M187AH188AM230AH239A from bacterial expression vector
Depositor
Christopher Counter
Article
Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1
Insert
MEK1 (MAP2K1 Human)
Tags
GST and HA
Mutation
M187A/H188A/M230A/H239A
Available Since
June 9, 2014
Availability
Available

Sequence Information

Length
1,105 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
HRV 3C site — recognition and cleavage site for human… 19..43 Fwd
DNA Sequence
CTGGAAGTTCTGTTCCAGGGGCCC
Protein Sequence
LEVLFQGP
HA — HA (human influenza hemagglutinin) epit… 70..97 Fwd
DNA Sequence
TACCCATACGATGTTCCAGATTACGCT
Protein Sequence
YPYDVPDYA