pET28a(+) 6xHis-TEV-IFIT5

53560 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
bacterial expression of IFIT5
Depositor
Kathleen Collins
Article
Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:
Insert
IFIT5 (IFIT5 Human)
Tags
6xHIS and TEV
Promoter
T7
Available Since
May 29, 2014
Availability
Available

Sequence Information

Length
1,099 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 167..185 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 194..212 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
TEV site — tobacco etch virus (TEV) protease recog… 227..248 Fwd
DNA Sequence
GAAAACCTGTATTTTCAGAGT
Protein Sequence
ENLYFQS
promoter 1
Label Gene Product Position Strand
T7 promoter — — 68..87 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 87..112 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC