ETS2A-c061

53619 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
bacterial expression plasmid
Depositor
Nicola Burgess-Brown
Insert
ETS2A (ETS2 Human)
Tags
His-6-TEV
Mutation
Bacterial expression for structure determination;…
Promoter
T7
Available Since
June 3, 2014
Availability
Available

Sequence Information

Length
1,004 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 51..69 Fwd
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 93..114 Fwd
DNA Sequence
GAGAACCTGTACTTCCAATCC
Protein Sequence
ENLYFQS
6xHis — 6xHis affinity tag 602..620 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 686..734 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG