pET-20b-T7-Ntx

58714 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expresses T7-Notexin in Escherichia coli
Depositor
Fiorella Tonello
Article
Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14
Insert
Notexin
Tags
T7 peptide tag
Promoter
T7
Available Since
Aug. 11, 2014
Availability
Available

Sequence Information

Length
778 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
Factor Xa site — Factor Xa recognition and cleavage site 55..67 Fwd
DNA Sequence
ATCGAAGGCCGC
Protein Sequence
IEGR
6xHis — 6xHis affinity tag 473..491 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 557..605 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG