YTC013-A06

58756 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
bacterial expression of PDB 4O2W
Depositor
Cheryl Arrowsmith
Insert
PDB:4O2W (HERC1 Human)
Tags
His
Mutation
Bacterial expression for structure determination;…
Promoter
T7
Available Since
Aug. 5, 2014
Availability
Available

Sequence Information

Length
887 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 49..67 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 79..100 Fwd
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG