JMC077-F02

58760 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
bacterial expression of PDB 4O64
Depositor
Cheryl Arrowsmith
Insert
PDB:4O64 (KDM2B Human)
Tags
His
Mutation
Bacterial expression for structure determination;…
Promoter
T7
Available Since
Aug. 5, 2014
Availability
Available

Sequence Information

Length
926 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 50..68 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 80..101 Fwd
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG
6xHis — 6xHis affinity tag 481..499 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 565..613 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG