PBC002-A04

58761 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
bacterial expression of PDB 4PWY
Depositor
Cheryl Arrowsmith
Insert
PDB:4PWY (PEX6 Human)
Tags
His
Mutation
Bacterial expression for structure determination;…
Promoter
T7
Available Since
Aug. 5, 2014
Availability
Available

Sequence Information

Length
944 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 20..38 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 50..71 Fwd
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG