SDC229-B03

58763 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
bacterial expression of PDB 4PYZ
Depositor
Cheryl Arrowsmith
Insert
PDB:4PYZ (USP7 Human)
Tags
His
Mutation
Bacterial expression for structure determination;…
Promoter
T7
Available Since
Aug. 5, 2014
Availability
Available

Sequence Information

Length
885 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 29..47 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 56..74 Fwd
DNA Sequence
CTGGTTCCGCGTGGTAGT
Protein Sequence
LVPRGS