pET14b_tev_PDZ2

59734 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
expresses PDZ2 domain of human ZO-1 in bacteria
Depositor
Alan Fanning
Article
Fanning et al J Biol Chem. 2007 Dec 28;282(52):37710-6
Insert
zonula occludens-1 PDZ2 (TJP1 Human)
Tags
HIS and TEV
Available Since
Sept. 11, 2014
Availability
Available

Sequence Information

Length
677 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
TEV site — tobacco etch virus (TEV) protease recog… 258..279 Rev
DNA Sequence
GAGAATCTTTATTTTCAGGGC
Protein Sequence
ENLYFQG
6xHis — 6xHis affinity tag 294..312 Rev
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 384..403 Rev
DNA Sequence
TAATACGACTCACTATAGG