pET14b_SG — PlasmidDB

Plasmid Information

Purpose

Expresses SH3-GUK domains of human ZO-1

Depositor

Alan Fanning

Article

Lye et al J Biol Chem. 2010 Apr 30;285(18):13907-1

Insert

zonula occludens-1 (TJP1 Human)

Length

803 bp

Topology

linear

Molecule Type

genomic DNA

CDS 2

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	142..160	Fwd
DNA Sequence CATCATCATCATCATCAC Protein Sequence HHHHHH
thrombin site	—	thrombin recognition and cleavage site	169..187	Fwd
DNA Sequence CTGGTGCCGCGCGGCAGC Protein Sequence LVPRGS

RBS 1

Label	Gene	Product	Position	Strand
Shine-Dalgarno sequence	—	—	345..354	Fwd
DNA Sequence TAAGGAGGT

promoter 1

Label	Gene	Product	Position	Strand
T7 promoter	—	—	51..70	Fwd
DNA Sequence TAATACGACTCACTATAGG