4LG6

60093 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
ANKRA:JMC023-D07:C211743 (ANKRA2 Human)
Tags
6xHis
Mutation
See comments
Promoter
T7
Available Since
Oct. 22, 2014
Availability
Available

Sequence Information

Length
723 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 34..52 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 64..85 Fwd
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG
6xHis — 6xHis affinity tag 630..648 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH