4MX2

60102 Synthetic Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
LdBPK_040440.1:MAC04A-B07:C226244
Tags
6xHis
Mutation
See comments
Promoter
T7
Available Since
Oct. 22, 2014
Availability
Available

Sequence Information

Length
772 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 33..51 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 63..84 Fwd
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG
Factor Xa site — Factor Xa recognition and cleavage site 114..126 Rev
DNA Sequence
ATCGAGGGGAGA
Protein Sequence
IEGR