M26I DJ-1 pET15b

60677 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Recombinant protein expression of Park7
Depositor
Mark Wilson
Article
Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.
Insert
M26I DJ-1 (PARK7 Human)
Tags
6xHis
Mutation
M26I
Promoter
T7
Available Since
Oct. 24, 2014
Availability
Available

Sequence Information

Length
714 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 41..59 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 68..86 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS