M26V DJ-1 pET15b

60688 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Recombinant protein expression of Park7
Depositor
Mark Wilson
Article
Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do
Insert
M26V DJ-1 (PARK7 Human)
Tags
6xHis
Mutation
M26V
Promoter
T7
Available Since
Oct. 24, 2014
Availability
Available

Sequence Information

Length
690 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 42..60 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 69..87 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS