pET15b-ITPA_P32T

60825 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
to purify human ITPA from bacteria
Depositor
Youri Pavlov
Article
Stepchenkova et al J Mol Biol. 2009 Sep 25;392(3):602-13. d
Insert
ITPA (ITPA Human)
Tags
His
Mutation
P32T
Promoter
T7
Available Since
March 9, 2015
Availability
Available

Sequence Information

Length
911 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 58..76 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 85..103 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS