pet42c(+)-GST-USP13

61749 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
express N-terminally GST tagged human USP13
Depositor
Yihong Ye
Article
Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife
Insert
USP13 (USP13 Human)
Tags
GST
Promoter
T7
Available Since
Feb. 5, 2015
Availability
Available

Sequence Information

Length
784 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 15..33 Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 42..60 Fwd
DNA Sequence
CTGGTGCCACGCGGTAGT
Protein Sequence
LVPRGS
S-Tag — affinity and epitope tag derived from p… 75..120 Fwd
DNA Sequence
AAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGC
Protein Sequence
KETAAAKFERQHMDS
Factor Xa site — Factor Xa recognition and cleavage site 150..162 Fwd
DNA Sequence
ATTGAGGGACGC
Protein Sequence
IEGR