pETDUET1-hUCH37 (ISF2)-NFRKB(39-156)

61938 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Co-expression of hUCH37 (ISF2) and hNFRKB (39-156) in E.coli
Depositor
Chris Hill
Article
VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1
Tags
6x HIS
Mutation
Construct ends at residue 156, residues 2-38 dele…
Promoter
T7
Available Since
Oct. 9, 2015
Availability
Available

Sequence Information

Length
805 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
7xHis — 6xHis affinity tag 145..166 Fwd
DNA Sequence
CATCACCACCATCATCATCAC
Protein Sequence
HHHHHHH
HRV 3C site — recognition and cleavage site for human… 166..190 Fwd
DNA Sequence
CTTGAAGTCCTCTTTCAGGGACCC
Protein Sequence
LEVLFQGP
promoter 1
Label Gene Product Position Strand
T7 promoter — — 46..65 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 65..90 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC