pET28A-p53 (1-73) (P27A)

62068 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expression of human p53 (1-73) (P27A) in e. coli
Depositor
Gary Daughdrill
Article
Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d
Insert
p53 (TP53 Human)
Tags
His
Mutation
Contains TAD residues 1-73; P27A
Promoter
T7
Available Since
Jan. 28, 2015
Availability
Available

Sequence Information

Length
682 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 32..50 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 59..77 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
6xHis — 6xHis affinity tag 311..329 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 395..443 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG