pET28-p53 (1-90) (72P)

62080 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expression of human p53 (1-90) (72P) in e. coli
Depositor
Gary Daughdrill
Article
Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d
Insert
p53 (TP53 Human)
Tags
His
Mutation
contains TAD/PP residues 1-90; 72P
Promoter
T7
Available Since
Jan. 29, 2015
Availability
Available

Sequence Information

Length
400 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 42..60 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 69..87 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
6xHis — 6xHis affinity tag 372..390 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH